CovidV1, Main, Exploration, bibRecord, 001565

Formation and activity of covalent conjugates of poliovirus and ligands binding to cell surface structures

Identifieur interne : 001565 ( Main/Exploration ); précédent : 001564; suivant : 001566

Formation and activity of covalent conjugates of poliovirus and ligands binding to cell surface structures

Auteurs : Christin Munkebye Aarnes [Norvège] ; Inger Helene Madshus [Norvège] ; Jean Claude Guillemot [Norvège] ; Kirsten Sandvig [Norvège] ; Sjur Olsnes [Norvège]

Source :

Experimental Cell Research [ 0014-4827 ] ; 1987.

RBID : ISTEX:3B27B2F33D7D4109ACB94A21E6B005653E03FA5A

English descriptors

Teeft :
- Ammonium acetate, Apical side, Asialoglycoprotein receptor, Biotin, Biotinylated, Biotinylated cells, Buoyant density, Cell surface, Cellular receptor, Cona, Conformational change, Conjugate, Diphtheria toxin, Free streptavidin, Hela, Hela ohio cells, Hydrophobic domains, Intact conjugate, Large number, Lysate, Microtitre plates, Natural poliovirus receptors, Olsnes, Poliovirus, Poliovirus receptors, Proc natl acad, Productive infection, Radioactive material, Receptor, Same buffer, Sandvig, Sendai virus, Specific binding, Streptavidin, Unbound conjugate, Unmodified, Unmodified cells, Unmodified virus, Unreacted streptavidin, Unreacted virus, Virus, Virus entry, Virus particle, Virus particles, Virus receptor, Virus surface.

Abstract

Abstract: Disulfide-linked conjugates of poliovirus with streptavidin or concanavalin A were formed and the binding of the conjugates to mouse L cells that lack natural poliovirus receptors was studied. The conjugate with streptavidin was specifically bound to biotinylated L cells, but not to unmodified L cells. The conjugate with conA was bound to L cells in the absence of, but not in the presence of α-methyl mannoside. Incubation of L cells with bound conjugates did not produce virus, although the conjugates were highly infectious in HeLa cells, containing natural poliovirus receptors. This suggests that the artificially bound virus was unable to penetrate the L cells and start replication. The possibility that binding of the virus to the natural receptor is required for efficient infection is discussed.

Url:

https://api.istex.fr/ark:/67375/6H6-BVM124HB-M/fulltext.pdf

DOI: 10.1016/0014-4827(87)90322-3

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 000553
to stream Istex, to step Curation: 000533
to stream Istex, to step Checkpoint: 000703
to stream Main, to step Merge: 001588
to stream Main, to step Curation: 001565

Le document en format XML

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<term>Biotinylated</term>
<term>Biotinylated cells</term>
<term>Buoyant density</term>
<term>Cell surface</term>
<term>Cellular receptor</term>
<term>Cona</term>
<term>Conformational change</term>
<term>Conjugate</term>
<term>Diphtheria toxin</term>
<term>Free streptavidin</term>
<term>Hela</term>
<term>Hela ohio cells</term>
<term>Hydrophobic domains</term>
<term>Intact conjugate</term>
<term>Large number</term>
<term>Lysate</term>
<term>Microtitre plates</term>
<term>Natural poliovirus receptors</term>
<term>Olsnes</term>
<term>Poliovirus</term>
<term>Poliovirus receptors</term>
<term>Proc natl acad</term>
<term>Productive infection</term>
<term>Radioactive material</term>
<term>Receptor</term>
<term>Same buffer</term>
<term>Sandvig</term>
<term>Sendai virus</term>
<term>Specific binding</term>
<term>Streptavidin</term>
<term>Unbound conjugate</term>
<term>Unmodified</term>
<term>Unmodified cells</term>
<term>Unmodified virus</term>
<term>Unreacted streptavidin</term>
<term>Unreacted virus</term>
<term>Virus</term>
<term>Virus entry</term>
<term>Virus particle</term>
<term>Virus particles</term>
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<term>Virus surface</term>
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<front><div type="abstract" xml:lang="en">Abstract: Disulfide-linked conjugates of poliovirus with streptavidin or concanavalin A were formed and the binding of the conjugates to mouse L cells that lack natural poliovirus receptors was studied. The conjugate with streptavidin was specifically bound to biotinylated L cells, but not to unmodified L cells. The conjugate with conA was bound to L cells in the absence of, but not in the presence of α-methyl mannoside. Incubation of L cells with bound conjugates did not produce virus, although the conjugates were highly infectious in HeLa cells, containing natural poliovirus receptors. This suggests that the artificially bound virus was unable to penetrate the L cells and start replication. The possibility that binding of the virus to the natural receptor is required for efficient infection is discussed.</div>
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<name sortKey="Guillemot, Jean Claude" sort="Guillemot, Jean Claude" uniqKey="Guillemot J" first="Jean Claude" last="Guillemot">Jean Claude Guillemot</name>
<name sortKey="Madshus, Inger Helene" sort="Madshus, Inger Helene" uniqKey="Madshus I" first="Inger Helene" last="Madshus">Inger Helene Madshus</name>
<name sortKey="Olsnes, Sjur" sort="Olsnes, Sjur" uniqKey="Olsnes S" first="Sjur" last="Olsnes">Sjur Olsnes</name>
<name sortKey="Sandvig, Kirsten" sort="Sandvig, Kirsten" uniqKey="Sandvig K" first="Kirsten" last="Sandvig">Kirsten Sandvig</name>
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Formation and activity of covalent conjugates of poliovirus and ligands binding to cell surface structures

Formation and activity of covalent conjugates of poliovirus and ligands binding to cell surface structures

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri